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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
14/05/2020 |
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Data da última atualização: |
14/05/2020 |
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Autoria: |
AGUIRRE, P. F.; GIACOMINI, S. J.; OLIVO, C. J.; BRATZ, V. F.; QUATRIN, M. P.; SCHAEFER, G. L. |
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Afiliação: |
Priscila Flôres Aguirre, Universidade Federal de Santa Maria - UFSM/Departamento de Zootecnia; Sandro José Giacomini, Universidade Federal de Santa Maria - UFSM/Departamento de Solos; Clair Jorge Olivo, Universidade Federal de Santa Maria - UFSM/Departamento de Zootecnia; Vinicius Felipe Bratz, Universidade Federal de Santa Maria - UFSM/Departamento de Zootecnia; Maurício Pase Quatrin, Universidade Federal de Santa Maria - UFSM/Departamento de Zootecnia; Gilmar Luiz Schaefer, Universidade Federal de Santa Maria - UFSM/Departamento de Solos. |
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Título: |
Biological nitrogen fixation and urea-N recovery in 'Coastcross-1' pasture treated with Azospirillum brasilense. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 55, e01242, 2020. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Fixação biológica de nitrogênio e recuperação de N-ureia em pastagem de 'Coastcross-1' tratada com Azospirillum brasilense. |
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Conteúdo: |
The objective of this work was to quantify the inoculation effect of Azospirillum brasilense (Ab-V5 and Ab-V6 strains) on the forage yield, biological nitrogen fixation (BNF), and urea-15N recovery of the forage grass 'Coastcross-1'. The experiment was carried out in a 2 (with or without inoculation) × 2 (without N fertilizer and with 100 kg ha-1 N per year as urea) × 7 (cuts) factorial arrangement. The natural 15N abundance method was used to determine BNF; to determine urea-N recovery, 15N-labeled urea was applied in microplots. Forage yield was higher in grasses subjected to inoculation, with 7.4 Mg ha-1 dry matter per year, for the treatment without N fertilizer, and 8.0 Mg ha-1 dry matter per year for the treatment with 100 kg ha-1 N per year, respectively, which shows an additive effect of inoculation and N fertilization. The contribution of BNF was 23.0 and 53.8 kg ha-1 per year for the unfertilized treatment, both in uninoculated and inoculated plants, respectively. Urea-15N recovery was 13.7 and 16.5 kg ha-1 per year for uninoculated and inoculated treatments, respectively, corresponding to 13.7 and 16.5% of the urea-N applied. Inoculation with A. brasilense increases forage yield and the contribution of BNF to grass nutrition with N, as well as urea-N recovery by the forage grass. |
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Palavras-Chave: |
Abundância natural de 15N; Natural abundance of 15N; Produção de forragem. |
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Thesagro: |
Cynodon Dactylon. |
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Thesaurus Nal: |
Forage yield. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213018/1/Biological-nitrogen-fixation-and-urea-N.pdf
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Marc: |
LEADER 02236naa a2200253 a 4500
001 2122327
005 2020-05-14
008 2020 bl uuuu u00u1 u #d
100 1 $aAGUIRRE, P. F.
245 $aBiological nitrogen fixation and urea-N recovery in 'Coastcross-1' pasture treated with Azospirillum brasilense.$h[electronic resource]
260 $c2020
500 $aTítulo em português: Fixação biológica de nitrogênio e recuperação de N-ureia em pastagem de 'Coastcross-1' tratada com Azospirillum brasilense.
520 $aThe objective of this work was to quantify the inoculation effect of Azospirillum brasilense (Ab-V5 and Ab-V6 strains) on the forage yield, biological nitrogen fixation (BNF), and urea-15N recovery of the forage grass 'Coastcross-1'. The experiment was carried out in a 2 (with or without inoculation) × 2 (without N fertilizer and with 100 kg ha-1 N per year as urea) × 7 (cuts) factorial arrangement. The natural 15N abundance method was used to determine BNF; to determine urea-N recovery, 15N-labeled urea was applied in microplots. Forage yield was higher in grasses subjected to inoculation, with 7.4 Mg ha-1 dry matter per year, for the treatment without N fertilizer, and 8.0 Mg ha-1 dry matter per year for the treatment with 100 kg ha-1 N per year, respectively, which shows an additive effect of inoculation and N fertilization. The contribution of BNF was 23.0 and 53.8 kg ha-1 per year for the unfertilized treatment, both in uninoculated and inoculated plants, respectively. Urea-15N recovery was 13.7 and 16.5 kg ha-1 per year for uninoculated and inoculated treatments, respectively, corresponding to 13.7 and 16.5% of the urea-N applied. Inoculation with A. brasilense increases forage yield and the contribution of BNF to grass nutrition with N, as well as urea-N recovery by the forage grass.
650 $aForage yield
650 $aCynodon Dactylon
653 $aAbundância natural de 15N
653 $aNatural abundance of 15N
653 $aProdução de forragem
700 1 $aGIACOMINI, S. J.
700 1 $aOLIVO, C. J.
700 1 $aBRATZ, V. F.
700 1 $aQUATRIN, M. P.
700 1 $aSCHAEFER, G. L.
773 $tPesquisa Agropecuária Brasileira$gv. 55, e01242, 2020.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Classificação |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/07/2007 |
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Data da última atualização: |
15/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
BONFIM, K.; FARIA, J. C.; NOGUEIRA, E. O. P. L.; MENDES, E. A; ARAGÃO, F. J. L. |
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Afiliação: |
Kenny Bonfim, Universidade de Brasília; Josias Correa de Faria, Embrapa Arroz e Feijão; Elsa Oliveira Paranagua e Lago Nogueira, Embrapa Recursos Genéticos e Biotecnologia; Érica de Azevedo Mendes bolsista Embrapa Recursos Genéticos e Biotecnologia; Francisco José Lima Aragão, Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
RNAI-Mediated resistance to bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris). |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Molecular Plant Microbe Interactions, v. 20, n.6, p.717-726, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Bean golden mosaic virus (BGMV) is transmitted by the whitefly Bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (Phaseolus vulgaris L.). The characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. The disease is the largest constraint to bean production in Latin America and causes severe yield losses (40 to 100%). Here, we explored the concept of using an RNA interference construct to silence the sequence region of the AC1 viral gene and generate highly resistant transgenic common bean plants. Eighteen transgenic common bean lines were obtained with an intron-hairpin construction to induce posttranscriptional gene silencing against the AC1 gene. One line (named 5.1) presented high resistance (approximately 93% of the plants were free of symptoms) upon inoculation at high pressure (more than 300 viruliferous whiteflies per plant during the whole plant life cycle) and at a very early stage of plant development. Transgene-specific small interfering RNAs were detected in both inoculated and noninoculated transgenic plants. A semiquantitative polymerase chain reaction analysis revealed the presence of viral DNA in transgenic plants exposed to viruliferous whiteflies for a period of 6 days. However, when insects were removed, no virus DNA could be detected after an additional period of 6 days. |
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Palavras-Chave: |
BGMV; Common bean; Geminivirus. |
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Thesagro: |
Feijão; Phaseolus Vulgaris; Resistência Genética; Vírus. |
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Thesaurus NAL: |
Bean golden mosaic virus; Begomovirus; RNA interference. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178163/1/ID-28711-1.pdf
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Marc: |
LEADER 02242naa a2200289 a 4500
001 1188929
005 2022-07-15
008 2007 bl uuuu u00u1 u #d
100 1 $aBONFIM, K.
245 $aRNAI-Mediated resistance to bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris).$h[electronic resource]
260 $c2007
520 $aBean golden mosaic virus (BGMV) is transmitted by the whitefly Bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (Phaseolus vulgaris L.). The characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. The disease is the largest constraint to bean production in Latin America and causes severe yield losses (40 to 100%). Here, we explored the concept of using an RNA interference construct to silence the sequence region of the AC1 viral gene and generate highly resistant transgenic common bean plants. Eighteen transgenic common bean lines were obtained with an intron-hairpin construction to induce posttranscriptional gene silencing against the AC1 gene. One line (named 5.1) presented high resistance (approximately 93% of the plants were free of symptoms) upon inoculation at high pressure (more than 300 viruliferous whiteflies per plant during the whole plant life cycle) and at a very early stage of plant development. Transgene-specific small interfering RNAs were detected in both inoculated and noninoculated transgenic plants. A semiquantitative polymerase chain reaction analysis revealed the presence of viral DNA in transgenic plants exposed to viruliferous whiteflies for a period of 6 days. However, when insects were removed, no virus DNA could be detected after an additional period of 6 days.
650 $aBean golden mosaic virus
650 $aBegomovirus
650 $aRNA interference
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aResistência Genética
650 $aVírus
653 $aBGMV
653 $aCommon bean
653 $aGeminivirus
700 1 $aFARIA, J. C.
700 1 $aNOGUEIRA, E. O. P. L.
700 1 $aMENDES, E. A
700 1 $aARAGÃO, F. J. L.
773 $tMolecular Plant Microbe Interactions$gv. 20, n.6, p.717-726, 2007.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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